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The World Anti-Doping Agency (WADA) published the 2025 List of Prohibited Substances and Methods, which was approved by WADA’s Executive Committee (ExCo) during its meeting on 12 September 2024 and enters into force on 1 January 2025

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Cysteine is one of the main amino acids required to synthesize glutathione (GSH), so depletion of cysteine concomitantly lowers the levels of glutathione in the host, in which GSH is a powerful antioxidant that plays a critical role in scavenging excess ROS and RNS (Fig