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Metformin did not affect general indicators of autophagy, nor specific mitophagy indicators in Y cells (Figures 4E–4H and 4J–4L), and also failed to impact age-related decreases in humanin and prohibitin (Figures S4A and S4B), two mitokines that regulate inflammation in some circumstances (Conte et al., 2019, Zapała et al., 2010, Kathiria et al., 2012)

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found that I/R was able to lead to liver damage, LPO as well as upregulation of the ferroptosis marker Ptgs2 in a murine model, and all of these effects were significantly alleviated by ferroptosis-specific inhibitor ferrostatin-1 and iron chelator deferoxamine [18]

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