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Ascorbic acid is a reducing agent that can reduce Cu(II) to Cu(I), potentially altering the copper coordination chemistry of GHK-Cu and reducing its biological activity

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Some samples were also supplemented with 10 μM L-DOPA to boost cell catecholamine production in the presence or absence of a monoamine oxidase inhibitor cocktail (10 μM: deprenyl, pargyline, clorgyline)