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Quantification of complement C3c fragment and IgG by flow cytometry Ten million ring- or trophozoite-infected and non-infected normal or GR-deficient RBCs were incubated for 30 min at room temperature in 50 µl of PBS-G, with or without the primary rabbit anti-human complement C3c fragment antibody or anti-human IgG antibody (Sigma, Milano, Italy), at a 1∶400 dilution, respectively